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Explain the principle of chromatography....

Explain the principle of chromatography.

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Chromatography has been developed as a method of separation components of a mixture gener ally between the stationary phase and a mobile phase.
Chromatography involves the three steps given below :
a) Adsorption and retention of a mixture of substances on the stationary phase and separation of adsorbed substances by the mobile phase to different distances on the stationary phase.
b) Recovery of the substances separated by a continuous flow of the mobile phase (known as elution) and
c) Qualitative and quantitative analysis of the eluted substances.
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