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Why is SDS used in polyacrylamide gel el...

Why is SDS used in polyacrylamide gel electrophoresis?

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A protein molecule contains many amino acids and all the amino acids do not have similar acid-base properties. Hence, a protein molecule cannot be conferred with uniform negative charges in a buffer solution.
It is therefore, treated with Sodium Dodecyl Sulphate (SDS), an anionic detergent that coats a protein molecule and confers negative charge to the protein uniformly.
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