Klenow fragment and Taq polymerase.

Klenow fragment does not possess

Which of the following steps are catalysed by Taq polymerase in a PCR reaction ? (a) Denaturation of template DNA (b) Annealing of primers to template DNA (c) Extension of primer and on the template DNA (d) All of the above

Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the high temperature induced denaturation of dsDNA. (ii) It requires primers for carrying out the process of polymerisation. (iii) It synthesises the RNA region between the primers, using dNTPs and Mg^(2+) .

Write note on Polymerases.

DNA fragments are :

Differentiate between restriction endonuclease and DNA polymerase.

Distinguish between:RNA polymerase and DNA polymerase

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes