During glycolysis, for each mole of glucose oxidised to pyruvate -

Options

A. 6 moles of ATP are produced
B. 2 moles of `NAD^(+)` are produced
C. 2 moles of ATP are used, and 4 moles of ATP are produced
D. No ATP is produced

Answer

### Step-by-Step Solution:

1. **Understanding Glycolysis**: Glycolysis is the metabolic pathway that converts glucose into pyruvate, producing energy in the form of ATP and NADH in the process.

2. **ATP Production**: During glycolysis, a total of 4 ATP molecules are produced through substrate-level phosphorylation. However, 2 ATP molecules are consumed in the initial steps of the glycolytic pathway.

3. **Net Gain of ATP**: To find the net gain of ATP during glycolysis, we subtract the ATP consumed from the ATP produced:
- ATP produced: 4
- ATP used: 2
- Net gain of ATP = 4 ATP produced - 2 ATP used = 2 ATP

4. **NADH Production**: In addition to ATP, glycolysis also produces NADH. Specifically, 2 molecules of NADH are generated during the conversion of glucose to pyruvate.

5. **Final Summary**: Thus, for each mole of glucose oxidized to pyruvate during glycolysis:
- 2 ATP are net gained.
- 2 NADH are produced.
- 4 ATP are produced in total, but 2 are used.

6. **Evaluating Options**: Now, let’s evaluate the provided options:
- **Option 1**: 6 moles of ATP are produced. (Incorrect)
- **Option 2**: 2 moles of NAD+ are produced. (Incorrect, NADH is produced, not NAD+)
- **Option 3**: 2 moles of ATP are used and 4 moles of ATP are produced. (Correct)
- **Option 4**: No ATP is produced. (Incorrect)

### Conclusion:
The correct answer is **Option 3**: 2 moles of ATP are used and 4 moles of ATP are produced.

---