Select the incorrect match .

Options

A. `{:("Column A","Colum B"),("Taq polymerase ","Annealing of DNA"):}`
B. `{:("Column A","Colum B"),("Chitinase ","Obtaining DNA by lysis of fungal cell"):}`
C. `{:("Column A","Colum B"),("Exonucleases ","Remove mucleotides from the ends of DNA"):}`
D. `{:("Column A","Colum B"),("DNA ligase ","Joining the fragments of DNA"):}`

Answer

To solve the question of selecting the incorrect match from the provided options, we will analyze each option step by step.

### Step-by-Step Solution:

1. **Understanding the Options**: We need to compare the items in Column 1 with their corresponding descriptions in Column 2 to identify any incorrect matches.

2. **Analyzing the First Option**:
- **Tagged Polymerase**: This is a heat-stable DNA polymerase derived from the thermophilic bacterium Thermus aquaticus.
- **Function**: It is primarily used in the Polymerase Chain Reaction (PCR) for the **extension** of primers on the DNA template.
- **Match Evaluation**: The option states that tagged polymerase is used for the **annealing** of DNA, which is incorrect. Therefore, this match is incorrect.

3. **Analyzing the Second Option**:
- **Chitinase**: This is an enzyme that breaks down chitin, a major component of the fungal cell wall.
- **Function**: By breaking down chitin, chitinase facilitates the lysis of fungal cells, making it easier to extract DNA.
- **Match Evaluation**: The option correctly states that chitinase is used for obtaining DNA by lysis of fungal cells. This match is correct.

4. **Analyzing the Third Option**:
- **Exonucleases**: These are enzymes that remove nucleotides from the ends of DNA.
- **Function**: They cleave nucleotides one at a time from the ends of a polynucleotide chain.
- **Match Evaluation**: The option correctly states that exonucleases remove nucleotides from the ends of DNA. This match is correct.

5. **Analyzing the Fourth Option**:
- **DNA Ligase**: This enzyme joins DNA fragments by catalyzing the formation of phosphodiester bonds.
- **Function**: It is essential for DNA replication and repair.
- **Match Evaluation**: The option correctly states that DNA ligase joins fragments of DNA. This match is correct.

6. **Final Conclusion**: After analyzing all options, the only incorrect match is the first one: **Tagged Polymerase and Annealing of DNA**. Therefore, the answer is that this is the incorrect match.

### Final Answer:
The incorrect match is **Tagged Polymerase and Annealing of DNA**.