Which of these enzymes are involved in the DNA isolation of fungi ?

Options

A. Pectinase
B. Cellulase
C. Chitinase
D. Ribonuclease

Answer

To solve the question regarding which enzymes are involved in the DNA isolation of fungi, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Cell Structure of Fungi**:
- Fungi have a cell wall that is primarily composed of chitin. This is a crucial point because the first step in DNA isolation is to break down the cell wall to access the DNA inside.

**Hint**: Remember that the cell wall composition is different in fungi compared to plants and bacteria.

2. **Identifying the Enzyme for Cell Wall Breakdown**:
- To break down the chitin in the fungal cell wall, the enzyme required is **chitinase**. This enzyme hydrolyzes chitin, allowing access to the cell contents, including the DNA.

**Hint**: Focus on the specific enzymes that target the components of the fungal cell wall.

3. **Considering Other Enzymes**:
- After breaking down the cell wall, other enzymes are necessary for complete DNA isolation:
- **Proteases**: These enzymes break down proteins that may be present in the cell, which is important to purify the DNA.
- **Ribonucleases**: These enzymes degrade RNA, which is also present in the cell and could interfere with the isolation of DNA.

**Hint**: Think about what other cellular components need to be removed to isolate pure DNA.

4. **Evaluating the Options**:
- **Pectinase**: This enzyme breaks down pectin, which is not a component of fungal cell walls, so it is not relevant here.
- **Cellulase**: This enzyme breaks down cellulose, which is found in plant cell walls, not fungal walls, making it irrelevant.
- **Chitinase**: This is the correct enzyme for breaking down the fungal cell wall.
- **Ribonucleases**: While important, they are not unique to fungi, so they do not specifically answer the question about enzymes involved in fungal DNA isolation.

5. **Conclusion**:
- The enzyme specifically involved in the DNA isolation of fungi is **chitinase**.

### Final Answer:
The correct enzyme involved in the DNA isolation of fungi is **Chitinase**.