The technique of protoplast fusion was developed by

Options

A. Helperin and Wetherell
B. Carlson at al
C. White
D. Steward.

Answer

**Step-by-Step Solution:**

1. **Understanding Protoplast Fusion**: Protoplast fusion is a technique used in plant biotechnology to create hybrid plants. It involves the fusion of protoplasts, which are plant cells that have had their cell walls removed.

2. **Identifying Key Scientists**: The question asks who developed this technique. The options provided are:
- A. Halperin and Wether
- B. Carson et al
- C. White and Stewart

3. **Historical Context**: Research indicates that the technique of protoplast fusion was developed in the early 1970s. It is important to identify the correct scientists associated with this development.

4. **Finding the Correct Answer**: The technique was specifically developed by Carson et al. in 1972. They were the first to create somatic hybrids from different species of the Nicotiana plant.

5. **Conclusion**: Based on the information gathered, the correct answer to the question is option B: Carson et al.

**Final Answer**: B. Carson et al.

---