First step in protoplasm fusion is

Options

A. Collection of somatic cell
B. Selection and isolation of somatic cells
C. Isolation of protoplasts
D. Hybridisation.

Answer

### Step-by-Step Solution:

1. **Understanding Protoplast Fusion**: Protoplast fusion is a technique used in plant biotechnology to create hybrid plants with desired traits. It involves the fusion of protoplasts, which are plant cells without their cell walls.

2. **Identifying the First Step**: The first step in the process of protoplast fusion is crucial. It involves preparing the cells that will be used for fusion.

3. **Options Analysis**:
- **A. Collection of somatic cells**: This step involves gathering the cells but does not specifically address the preparation needed for fusion.
- **B. Selection and isolation of somatic cells**: This step involves not only collecting but also isolating the specific somatic cells that will be used to obtain protoplasts.
- **C. Isolation of protoplast**: This step occurs after the somatic cells have been selected and isolated.
- **D. Hybridization**: This is the final step where the actual fusion occurs after protoplasts have been prepared.

4. **Conclusion**: The correct first step in protoplast fusion is **B. Selection and isolation of somatic cells**. This step is essential as it ensures that the right cells are chosen and prepared for the subsequent isolation of protoplasts.