Axenic culture is

Options

A. Culture of callus
B. Culture containing embryoids
C. Culture devoid of nutrients
D. Culture without contamination.

Answer

### Step-by-Step Solution:

1. **Understanding the Term "Axenic Culture":**
- Axenic culture refers to a culture that is free from any other living organisms except for the one being studied. This means that the culture is pure and does not contain any contaminants.

2. **Analyzing the Options:**
- A) Culture of callus: This option refers to a mass of cells that can be grown in tissue culture, but it does not imply the absence of other organisms.
- B) Culture containing embryos: This option is specific to embryonic cultures and does not indicate purity or contamination.
- C) Culture devoid of nutrients: This option suggests a lack of nutrients, which is not related to the concept of axenic cultures.
- D) Culture without contamination: This option accurately describes an axenic culture as it indicates that the culture is free from other microorganisms.

3. **Identifying the Correct Answer:**
- Based on the definitions and the analysis of the options, the correct answer is D) culture without contamination.

### Final Answer:
The correct answer is D) culture without contamination.