Endonuclease is employed in

Options

A. Transcription
B. Translation
C. Genetic engineering
D. DNA replication

Answer

**Step-by-Step Solution:**

1. **Understanding Endonucleases**: Endonucleases are enzymes that cut DNA at specific sites within the molecule. This ability is crucial for various molecular biology techniques.

2. **Identifying the Processes**: The question asks in which processes endonucleases are employed. The main processes to consider are:
- Transcription (DNA to RNA)
- Translation (RNA to Protein)
- DNA Replication (DNA to DNA)

3. **Analyzing Each Process**:
- **Transcription**: This process involves copying a segment of DNA into RNA. Endonucleases are not required here since the DNA is not cut.
- **Translation**: This is the process where RNA is translated into proteins. Again, endonucleases are not involved as this does not require cutting DNA.
- **DNA Replication**: This process involves the duplication of DNA. While it involves unwinding and separating DNA strands, it does not require cutting the DNA.

4. **Identifying the Correct Application**: Endonucleases are primarily used in genetic engineering, where they are employed to cut DNA at specific sites. This allows for the insertion of new genes or modification of existing ones.

5. **Conclusion**: The correct answer to the question is that endonucleases are employed in genetic engineering, particularly in recombinant DNA technology, where they are used to cut DNA at palindromic sites.

**Final Answer**: Endonucleases are employed in genetic engineering.

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