Identify the plasmid...

Identify the plasmid

EcoRI

pbR 322

BamH I

Hind III

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A plasmid

A plasmid is

Plasmid is

Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to - DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments . (v) Use ligase to seal plasmid DNA to non- plasmid DNA Arrange the above steps in the right sequence and select the CORRECT option

Identify the type of vector represented in the diagram given below. (a) An expressable plasmid (b) A tumor inducing plasmid (c) Plasmid which expresses in two hosts (d) The plasmid to be constructed

I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacteria cells. IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligate to seal plasmid DNA to non-plasmid DNA. From the given list, which of the following is the most logical sequence of steps for splicing foregain DNA into a plasmid and inserting the plasmid into a bacterium ?

Which of the following is not a feature of the plasmids ?

Identify the desirable characteristics for a plasmid used in rDNA technology from the following (a) Ability to multiply and express outside the host in a bioreactor (b) A highly active promoter (c) A site at which replication can be initiated (d) One or more identifiable marker genes (e) One or more unique restriction sites

DINESH PUBLICATION ENGLISH-PRINCIPLES AND APPLICATIONS OF BIOTECHNOLOGY-RQ

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