Which is employed for synthesis monoclonal antibody by hybridisation technique

Options

A. RBC
B. Liver cells
C. Tumour cells
D. Nerve cells

Answer

**Step-by-Step Solution:**

1. **Understanding Monoclonal Antibodies**: Monoclonal antibodies are identical antibodies produced by a single clone of cells. They are used in various applications, including diagnostics and therapeutics.

2. **Hybridoma Technology**: This is the technique used for the production of monoclonal antibodies. It involves the fusion of two different types of cells to create a hybrid cell that can produce antibodies.

3. **Selection of Cells**: The two types of cells used in hybridoma technology are:
- **Spleen Cells**: These are derived from mice that have been immunized with a specific antigen. They are responsible for producing the desired antibodies.
- **Myeloma Cells**: These are tumor cells that have the ability to grow indefinitely in culture. They do not produce antibodies but can be fused with spleen cells to create hybrid cells.

4. **Fusion Process**: The spleen cells and myeloma cells are fused using a substance like polyethylene glycol. This process creates hybrid cells known as hybridomas.

5. **Screening and Selection**: The hybridomas are then screened to identify those that produce the desired monoclonal antibody. The selected hybridomas can be cultured to produce large quantities of the antibody.

6. **Conclusion**: The correct answer to the question is that **tumor cells** (myeloma cells) are employed for the synthesis of monoclonal antibodies by the hybridization technique.

**Final Answer**: Tumor cells

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