Insect tolerant gene from Bacilus thuringiensis is introduced using Ti plasmid of

Options

A. Escherichia coli
B. Agrobacterium tumefaciens
C. Haemphilus influenzae
D. Arabidopsis thaliana

Answer

To solve the question about the introduction of the insect-tolerant gene from Bacillus thuringiensis using Ti plasmid, we can follow these steps:

### Step-by-Step Solution:

1. **Identify the Source of the Gene**:
- The insect-tolerant gene is derived from Bacillus thuringiensis (Bt). This gene is often referred to as the BT gene or cry gene, which produces a protein toxin harmful to certain insects.

2. **Understand the Role of Ti Plasmid**:
- Ti plasmid (Tumor-inducing plasmid) is a type of plasmid that can be used as a vector to introduce foreign genes into plant cells. It is essential for genetic engineering in plants.

3. **Determine the Origin of Ti Plasmid**:
- The Ti plasmid is specifically found in Agrobacterium tumefaciens, a bacterium known for its ability to transfer DNA to plant cells, causing tumors in plants.

4. **Link the Gene and the Vector**:
- The BT gene from Bacillus thuringiensis can be inserted into the Ti plasmid. This Ti plasmid then serves as a vector to carry the BT gene into the plant cells, allowing for the expression of insect tolerance in the plants.

5. **Select the Correct Answer**:
- Based on the information gathered, the correct answer to the question is **Agrobacterium tumefaciens**, as it is the source of the Ti plasmid used to introduce the insect-tolerant gene.

### Final Answer:
The insect-tolerant gene from Bacillus thuringiensis is introduced using Ti plasmid of **Agrobacterium tumefaciens**.

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