Biolistic technique is used in :

Options

A. Tissue culture process
B. Hybridisation process
C. Germplasm conservation process
D. Gene transfer process

Answer

**Step-by-Step Solution:**

1. **Understanding Biolistic Technique**: The biolistic technique, also known as the "gene gun" method, is a method used to introduce foreign DNA into cells. It involves the use of microscopic particles that are coated with DNA.

2. **Mechanism of Action**: In this technique, DNA-coated microscopic particles (often gold or tungsten) are accelerated and then shot into the target cells using a device that functions like a gun. This allows for the direct delivery of genetic material into the cells.

3. **Applications of Biolistic Technique**: The primary application of the biolistic technique is in the gene transfer process. This is crucial for genetic engineering, where scientists aim to modify the genetic makeup of organisms.

4. **Exclusion of Other Options**:
- **Tissue Culture Process**: This involves growing cells or tissues in an artificial medium but does not specifically involve the introduction of foreign DNA.
- **Hybridization Process**: This refers to the crossing of different species or varieties to produce hybrids, which is not related to the biolistic technique.
- **Germplasm Conservation Process**: This involves preserving genetic material but does not involve the active transfer of genes into cells.

5. **Conclusion**: Based on the explanation and the mechanism of the biolistic technique, it is clear that the correct answer to the question is that the biolistic technique is used in the gene transfer process.

**Final Answer**: The biolistic technique is used in the gene transfer process.

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