In hybridoma technolgy

Options

A. B-cells are fused with myeloma cells
B. T-cells are fused with myeloma cells
C. T-cells are fused with B-cells
D. None of the above

Answer

### Step-by-Step Solution:

1. **Understanding Hybridoma Technology**:
- Hybridoma technology is a method used to produce large quantities of monoclonal antibodies. It involves the fusion of specific cells to create hybrid cells that can produce antibodies.

2. **Identifying the Key Cells**:
- The key cells involved in hybridoma technology are B-cells and myeloma cells. B-cells are responsible for producing antibodies, while myeloma cells are cancerous cells that can divide indefinitely.

3. **The Fusion Process**:
- In hybridoma technology, B-cells (which produce antibodies) are fused with myeloma cells (which can replicate indefinitely). This fusion creates hybrid cells, known as hybridomas.

4. **Capabilities of Hybridomas**:
- The hybridomas generated from this fusion possess two important capabilities:
- They can produce the desired antibodies (from the B-cells).
- They can replicate indefinitely (from the myeloma cells).

5. **Conclusion**:
- Therefore, the correct statement regarding hybridoma technology is that B-cells are fused with myeloma cells to create hybridomas that produce monoclonal antibodies.

### Final Answer:
The correct option is: **B-cells are fused with myeloma cells.**