Which is correctly matched

Options

A. Agrobacterium tumefaciens - Tumour
B. pBR 322-Enzyme
C. Thermus aquaticus-Bt gene
D. Ligase - Molecular scissors

Answer

To solve the question "Which is correctly matched?" we need to analyze each option provided and determine if the pairs are accurately related.

### Step-by-Step Solution:

1. **Evaluate the First Option: Agrobacterium tumefaciens - Tumor**
- Agrobacterium tumefaciens is a soil bacterium known for causing crown gall disease in plants. This disease results in the formation of tumors on the plant.
- **Conclusion:** This option is correctly matched.

2. **Evaluate the Second Option: PBR322 - Enzyme**
- PBR322 is a plasmid that was one of the first cloning vectors used in E. coli. It is not an enzyme; rather, it is a genetic element used for cloning.
- **Conclusion:** This option is incorrectly matched.

3. **Evaluate the Third Option: Thermus aquaticus - Bt gene**
- Thermus aquaticus is a bacterium that thrives in high temperatures and is known for producing Taq polymerase, an enzyme used in PCR (Polymerase Chain Reaction). The Bt gene, however, is derived from Bacillus thuringiensis, which is used for pest control in plants.
- **Conclusion:** This option is incorrectly matched.

4. **Evaluate the Fourth Option: Ligase - Molecular scissors**
- Ligase is an enzyme that joins DNA strands together. In contrast, molecular scissors refer to restriction enzymes that cut DNA at specific sequences. Therefore, ligase does not function as molecular scissors.
- **Conclusion:** This option is incorrectly matched.

### Final Answer:
The only correctly matched pair is **Option 1: Agrobacterium tumefaciens - Tumor**.

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