Gene amplification using primers can be done by :

Options

A. Microinjection
B. ELISA
C. Polymerase chain reaction
D. Gene gun

Answer

To answer the question "Gene amplification using primers can be done by:", we will analyze each of the provided options and determine which one is correct.

### Step-by-Step Solution:

1. **Understanding the Options**:
- The options provided are: Microinjection, ELISA, Polymerase Chain Reaction (PCR), and Gene Gun.

2. **Analyzing ELISA**:
- ELISA stands for Enzyme-Linked Immunosorbent Assay. It is primarily used for detecting and quantifying proteins, peptides, antibodies, and hormones. It does not involve gene amplification or the use of primers.
- **Conclusion**: ELISA is not the correct answer.

3. **Analyzing Gene Gun**:
- A Gene Gun is a device used to introduce DNA into cells by shooting microscopic gold or tungsten particles coated with DNA into the cells. While it facilitates the transfer of DNA, it does not specifically amplify genes using primers.
- **Conclusion**: Gene Gun is not the correct answer.

4. **Analyzing Microinjection**:
- Microinjection involves the direct injection of genetic material into a living cell using a fine glass micropipette. This method allows for the introduction of DNA but does not inherently amplify genes using primers.
- **Conclusion**: Microinjection is not the correct answer.

5. **Analyzing Polymerase Chain Reaction (PCR)**:
- The Polymerase Chain Reaction (PCR) is a widely used technique in molecular biology to amplify specific DNA sequences. It involves the use of short single-stranded DNA sequences called primers, which bind to the target DNA and initiate the replication process. This method allows for the exponential amplification of the target gene.
- **Conclusion**: PCR is the correct answer as it specifically uses primers for gene amplification.

### Final Answer:
The correct answer is **Polymerase Chain Reaction (PCR)**.

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