Biolistics (gene-gun) is suitable for...

Biolistics (gene-gun) is suitable for

Transformation of plant cells

Disarming pathogen vectors

DNA finger printing

Constructing recombinant DNA

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Which statement is suitable for tet^(R) gene

A direct method of gene transfer which is suitable for plants, in this cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA is ?

Biolistic technique is used in :

Assertion: In biolistics method, cells are bombarded with high velocity micr-particles of gold or tungsten coated with DNA Reason :It is suitable for plant cells

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Statement - 1 : Biolistics method of gene transfer is an example of direct gene transfer. Statement - 2 : In biolistics method , pBR322 is used.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Mention the type of host cells suitable for the gene guns to introduce an alien DNA.

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