Amplification of gene of interest by using DNA polymerase may go upto

Options

A. 0.1 million
B. 1.0 million
C. 1.0 billion
D. 1.0 trollion

Answer

To solve the question regarding the amplification of a gene of interest using DNA polymerase, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding PCR**: The Polymerase Chain Reaction (PCR) is a technique used in molecular biology to amplify a specific segment of DNA. It allows for the creation of numerous copies of a particular DNA sequence.

**Hint**: PCR is essential for amplifying DNA segments for various applications in research and medicine.

2. **Mechanism of Amplification**: During PCR, the DNA is subjected to cycles of denaturation, annealing, and extension. In each cycle, the amount of DNA doubles.

**Hint**: Remember that the doubling effect is a key feature of the PCR process.

3. **Calculating Copies After n Cycles**: The formula for the number of copies after n cycles is given by \(2^n\), where n is the number of cycles.

**Hint**: Think about how exponential growth works; each cycle doubles the amount of DNA.

4. **Typical Number of Cycles**: In standard PCR protocols, the number of cycles is typically around 30.

**Hint**: Consider the common practices in laboratory settings when determining the number of cycles.

5. **Calculating Total Copies**: If we use 30 cycles, we can calculate the total number of copies:
\[ \text{Total Copies} = 2^{30} \]
This results in approximately 1 billion copies (specifically, \(1,073,741,824\) copies).

**Hint**: Use a calculator or a power of two table to find \(2^{30}\) if needed.

6. **Conclusion**: Therefore, the amplification of a gene of interest using DNA polymerase may go up to approximately 1 billion copies.

**Hint**: Always round your final answer to match the options provided in the question.

### Final Answer:
The amplification of gene of interest by using DNA polymerase may go up to **1 billion** copies.