The technique used to make numerous copies of a specific segment of DNA quickly and accurately is

Options

A. Ligase chain reaction
B. Transcription
C. Polymerase chain reaction
D. Translation

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for a technique that allows for the rapid and accurate copying of a specific segment of DNA.

2. **Identifying Key Steps in DNA Copying**: To copy DNA, there are three essential steps involved:
- **Denaturation**: This is the first step where the double-stranded DNA is heated to separate it into two single strands.
- **Annealing**: In this step, short DNA primers bind to the single-stranded DNA at specific locations, preparing for the next step.
- **Extension**: The final step where the enzyme DNA polymerase synthesizes new DNA strands by adding nucleotides to the primers.

3. **Recognizing the Technique**: The combination of these three steps is known as the Polymerase Chain Reaction (PCR). PCR is a widely used method in molecular biology to amplify specific DNA segments.

4. **Evaluating the Options**: The question provides several options:
- **Ligase Chain Reaction**: This is not a recognized technique for DNA amplification.
- **Transcription**: This process involves synthesizing mRNA from DNA, not copying DNA.
- **Polymerase Chain Reaction (PCR)**: This is the correct answer, as it is specifically designed for amplifying DNA.
- **Translation**: This is the process of converting mRNA into protein, which is unrelated to DNA copying.

5. **Final Answer**: The technique used to make numerous copies of a specific segment of DNA quickly and accurately is the **Polymerase Chain Reaction (PCR)**.

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