Which statement is suitable for `tet^(R)` gene

Options

A. It has Pst - I recognition site
B. It has Bam HI and Sal-I recognition sites
C. It has tet III recognition sites
D. None of the above

Answer

To determine which statement is suitable for the `tet^(R)` gene (tetracycline resistance gene), we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Context**: The `tet^(R)` gene is associated with tetracycline resistance. It is important to identify the characteristics of this gene, particularly in the context of the PBR322 plasmid.

2. **Evaluate the Options**: The question presents several statements regarding the `tet^(R)` gene. We need to analyze each statement to determine its validity.

3. **Analyze the First Statement**: The first statement claims that the `tet^(R)` gene has a PST1 recognition site.
- **Check the PBR322 Diagram**: According to the information, the PST1 recognition site is associated with the ampicillin resistance gene, not the tetracycline resistance gene.
- **Conclusion**: This statement is incorrect.

4. **Analyze the Second Statement**: The second statement claims that the `tet^(R)` gene has BAMH1 and SAL1 recognition sites.
- **Check the PBR322 Diagram Again**: The diagram indicates that the `tet^(R)` gene is indeed located between the BAMH1 and SAL1 recognition sites.
- **Conclusion**: This statement is correct.

5. **Analyze the Third Statement**: The third statement claims that the `tet^(R)` gene has TET3 recognition sites.
- **Check the Information**: There is no mention of TET3 recognition sites being associated with the `tet^(R)` gene in the context provided.
- **Conclusion**: This statement is incorrect.

6. **Final Conclusion**: Based on the analysis, the correct statement regarding the `tet^(R)` gene is that it has BAMH1 and SAL1 recognition sites.

### Final Answer:
The suitable statement for the `tet^(R)` gene is that it has BAMH1 and SAL1 recognition sites.

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