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E. coli having plasmids for production o...

E. coli having plasmids for production of human insulin is cultured in medium containing

A

Glucose

B

Maltose

C

Lactose

D

Sucrose

Text Solution

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The correct Answer is:
### Step-by-Step Solution 1. **Understanding the Organism**: The question refers to *E. coli* that has been genetically modified to produce human insulin. This *E. coli* is termed a transgenic organism because it has been engineered to contain a human gene responsible for insulin production. 2. **Plasmid Insertion**: The human insulin gene is inserted into a plasmid, which is a small, circular piece of DNA that exists independently of the chromosomal DNA in bacteria. Plasmids can replicate independently and are often used in genetic engineering to introduce new genes into bacteria. 3. **Choosing the Culture Medium**: To culture this genetically modified *E. coli*, a suitable growth medium is required. The medium must provide the necessary nutrients for the bacteria to grow and express the inserted gene. 4. **Role of Lactose**: The best choice for the culture medium in this case is lactose. This is because *E. coli* has a natural system known as the lac operon, which is activated in the presence of lactose. The lac operon allows the bacteria to metabolize lactose, leading to the production of glucose and galactose. 5. **Why Not Glucose or Galactose?**: If glucose or galactose were provided instead of lactose, the lac operon would not be activated. This is because the presence of glucose inhibits the expression of the lac operon, preventing the bacteria from utilizing the plasmid effectively for insulin production. 6. **Conclusion**: Therefore, to ensure that the *E. coli* can effectively produce human insulin, it should be cultured in a medium containing lactose. ### Final Answer *E. coli having plasmids for the production of human insulin is cultured in a medium containing lactose.* ---
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