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In the technique of recombinant insulin ...

In the technique of recombinant insulin production, the genes for `alpha`- and `beta`- polypeptides were inserted into the plasmid by the side of

A

Antibiotic resistance gene

B

Lac Z promoter gene

C

`beta`- galactosidase gene

D

Ori

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The correct Answer is:
To solve the question regarding the insertion of the genes for alpha- and beta-polypeptides in the recombinant insulin production technique, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding the Context**: - Initially, insulin was derived from animal sources, which caused allergic reactions in some humans. To address this, recombinant DNA technology was developed to produce human insulin using bacteria. 2. **Identifying the Organism**: - The bacterium used for producing recombinant insulin is *Escherichia coli* (E. coli). This bacterium is chosen for its ability to replicate quickly and efficiently. 3. **Understanding Plasmids**: - A plasmid is a small, circular piece of DNA that is separate from the chromosomal DNA in bacteria. Plasmids can replicate independently and are often used in genetic engineering to transfer genes. 4. **Insertion of Genes**: - The genes for the alpha and beta polypeptides of insulin need to be inserted into the plasmid. This is done to ensure that when the plasmid replicates, it also replicates the genes for insulin. 5. **Identifying the Insertion Site**: - The insertion of the insulin genes should not disrupt essential functions of the plasmid. The ideal site for insertion is the **origin of replication (ORI)**. This site allows the plasmid to replicate independently of the bacterial chromosome. 6. **Conclusion**: - Therefore, the correct answer to the question is that the genes for alpha- and beta-polypeptides were inserted into the plasmid by the site known as the **origin of replication (ORI)**. ### Final Answer: The genes for alpha- and beta-polypeptides were inserted into the plasmid by the site known as the **origin of replication (ORI)**. ---
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