Products of restriction enzyme digestion are separated by

Options

A. Agarose gel electrophoresis
B. Centrifugation
C. Polyacrylamide gel electrophoresis
D. PCR

Answer

To answer the question "Products of restriction enzyme digestion are separated by," we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Restriction Enzymes**:
- Restriction enzymes, also known as restriction endonucleases, cut DNA at specific sequences. This results in various DNA fragments of different sizes.

2. **Purpose of Separation**:
- After digestion, it is essential to separate these DNA fragments to analyze their size and quantity. This separation allows researchers to visualize and study the DNA fragments.

3. **Choosing a Separation Method**:
- The most common method for separating DNA fragments is electrophoresis. This technique uses an electric field to move charged particles through a medium.

4. **Selecting the Gel Type**:
- There are different types of gels used for electrophoresis, such as agarose and polyacrylamide. For separating larger DNA fragments, agarose gel electrophoresis is preferred.

5. **Understanding Agarose Gel Electrophoresis**:
- In agarose gel electrophoresis, the gel acts as a molecular sieve. When an electric current is applied, the negatively charged DNA fragments move towards the positive electrode. Smaller fragments move faster and farther than larger ones.

6. **Conclusion**:
- Therefore, the products of restriction enzyme digestion are separated by **agarose gel electrophoresis**.

### Final Answer:
The products of restriction enzyme digestion are separated by **agarose gel electrophoresis**.

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