RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in preventing the process of:

Options

A. Transcription
B. Replication of DNA
C. Translation of mRNA
D. Splicing of hnRNA

Answer

To solve the question regarding RNA interference (RNAi) and its role in making tobacco plants resistant to Meloidogyne incognita, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding RNA Interference (RNAi)**:
- RNA interference is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules.

2. **Role of Double-Stranded RNA (dsRNA)**:
- In RNAi, double-stranded RNA (dsRNA) is introduced into the plant cells. This dsRNA is processed into small interfering RNA (siRNA).

3. **Formation of RNA-Induced Silencing Complex (RISC)**:
- The siRNA then assembles into a complex known as the RNA-induced silencing complex (RISC). This complex is crucial for the silencing process.

4. **Targeting mRNA**:
- The siRNA within the RISC guides the complex to complementary mRNA molecules. When the RISC binds to these target mRNAs, it leads to their cleavage and degradation.

5. **Consequences of mRNA Cleavage**:
- The degradation of target mRNA means that it cannot be translated into proteins. Therefore, the expression of the gene corresponding to that mRNA is effectively silenced.

6. **Identifying the Process Prevented**:
- Since the primary outcome of RNA interference is the destruction of mRNA, which prevents it from being translated into proteins, we conclude that RNAi prevents the process of translation of mRNA.

### Final Answer:
RNA interference, which is employed in making tobacco plants resistant to Meloidogyne incognita, is essentially involved in preventing the process of **translation of mRNA**.

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