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In pBR 322, the gene sequence that codes...

In pBR 322, the gene sequence that codes for the proteins involved in replication of the plasmid is

A

Pvu II

B

Cla I

C

rop

D

pst I

Text Solution

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The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding pBR322**: pBR322 is an artificial cloning vector developed by Bolivar and Rodrigues. It is named after them, where 'B' stands for Bolivar and 'R' for Rodrigues. 2. **Identifying Components**: pBR322 contains several important components, including selectable markers for antibiotic resistance (ampicillin and tetracycline), multiple cloning sites (MCS), and an origin of replication. 3. **Role of the Origin of Replication**: The origin of replication is crucial as it determines the copy number of the plasmid within the host cell. It is responsible for initiating the replication process. 4. **Identifying the Gene for Replication**: Within pBR322, the gene sequence that codes for the proteins involved in the replication of the plasmid is referred to as ROP (Replication of Plasmid). ROP is essential for the stability and replication of the plasmid. 5. **Conclusion**: Therefore, the gene sequence that codes for the proteins involved in the replication of the plasmid in pBR322 is ROP. **Final Answer**: The gene sequence that codes for the proteins involved in the replication of the plasmid in pBR322 is ROP. ---
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