Cry ' gene is obtained from:

Options

A. Agrobacterium tumefaciens
B. Bacillus thuringiensis
C. Rhizopus leguminosarum
D. Rhizobium phaseoli

Answer

**Step-by-Step Solution:**

1. **Understanding the Cry Gene**: The cry gene is known for its role in producing insecticidal proteins, specifically cryoproteins, which are toxic to certain insects.

2. **Identifying the Source**: The question asks from which bacteria the cry gene is obtained. The options provided include:
- Agrobacterium tumefaciens
- Bacillus thuringiensis
- Rhizopus leguminosarum
- Rhizobium fazoli

3. **Analyzing Each Option**:
- **Agrobacterium tumefaciens**: This bacterium is known as a natural genetic engineer and is responsible for crown gall tumors in dicot plants. It does not produce insecticidal proteins, so it is not the source of the cry gene.
- **Bacillus thuringiensis (Bt)**: This bacterium produces the cry gene, which encodes for cryoproteins that are toxic to insects. This is the correct answer.
- **Rhizopus leguminosarum**: This is a fungus and does not produce cry proteins, hence not the source of the cry gene.
- **Rhizobium fazoli**: This is a bacterium associated with nitrogen fixation in legumes and does not produce cry proteins, making it an incorrect option as well.

4. **Conclusion**: The cry gene is obtained from **Bacillus thuringiensis** (Bt), which is widely used in biotechnology for its insecticidal properties.

**Final Answer**: The cry gene is obtained from **Bacillus thuringiensis**.

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