The first recombinant DNA was constructed by linking an antibiotic resistant gene with the native plasmid of

Options

A. Escherichia coli
B. Salmonella typhimurium
C. Clostridium butylicum
D. Acetobacter aceti

Answer

To solve the question regarding the first recombinant DNA constructed by linking an antibiotic-resistant gene with a native plasmid, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Concept of Recombinant DNA**:
- Recombinant DNA technology involves combining DNA from different sources. This is often done to introduce new traits or characteristics into an organism.

2. **Identify Key Contributors**:
- The first successful construction of recombinant DNA was achieved by scientists Stanley Cohen and Herbert Boyer in 1972.

3. **Determine the Components Used**:
- The process involved linking an antibiotic-resistant gene to a plasmid. A plasmid is a small, circular piece of DNA commonly found in bacteria.

4. **Identify the Bacterium**:
- The specific bacterium used in this pioneering work was Salmonella typhimurium. This bacterium's native plasmid was utilized for the recombinant DNA construction.

5. **Conclude the Answer**:
- Therefore, the first recombinant DNA was constructed by linking an antibiotic-resistant gene with the native plasmid of **Salmonella typhimurium**.

### Final Answer:
The first recombinant DNA was constructed by linking an antibiotic-resistant gene with the native plasmid of **Salmonella typhimurium**.

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