Select the wrong statement
(a) Presence of chromogenic substrate gives blue color colonies if the plasmid in the bacteria does not have an insert
(b) Retroviruses in animals have the ability to transform normal cells into cancerous cells
(c) In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
(d) Since DNA is a hydrophilic molecule it cannot pass through cell membranes

Options

A. Presence of chromogenic substrate gives blue colour colonies if the plasmid in the bacteria does not have an insert
B. Retroviruses in animals have the ability to transform normal cells into cancerous cells
C. In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
D. Since DNA is a hydrophilic molecule it cannot pass through cell membranes

Answer

To solve the question of selecting the wrong statement, we will analyze each option one by one.

### Step-by-Step Solution:

1. **Option (a)**: "Presence of chromogenic substrate gives blue color colonies if the plasmid in the bacteria does not have an insert."
- **Analysis**: This statement is correct. In molecular cloning, when a plasmid containing a gene for β-galactosidase is transformed into bacteria, the presence of a chromogenic substrate (like X-gal) leads to blue colonies if the plasmid does not have an insert (non-recombinant). If the plasmid has an insert (recombinant), the colonies appear white.
- **Conclusion**: This statement is true.

2. **Option (b)**: "Retroviruses in animals have the ability to transform normal cells into cancerous cells."
- **Analysis**: This statement is also correct. Retroviruses can integrate their genetic material into the host cell's DNA, which can lead to the transformation of normal cells into cancerous cells. This is a well-documented phenomenon in virology.
- **Conclusion**: This statement is true.

3. **Option (c)**: "In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA."
- **Analysis**: This statement is incorrect. The process described here refers to the "gene gun" method, not microinjection. Microinjection involves directly injecting DNA into the cell using a fine glass pipette, rather than bombarding the cells with particles.
- **Conclusion**: This statement is false.

4. **Option (d)**: "Since DNA is a hydrophilic molecule it cannot pass through cell membranes."
- **Analysis**: This statement is correct. DNA is indeed hydrophilic and cannot easily pass through the hydrophobic lipid bilayer of cell membranes without assistance (like through transfection methods).
- **Conclusion**: This statement is true.

### Final Answer:
The wrong statement is **(c)**.