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With respect to DNA fragmentation Stat...

With respect to DNA fragmentation
Statement A: Gel electrophoresis and elution are two important processes
Statement B : After staining with ethidium bromide, it has to be exposed to UV light

A

Only A is correct and B is not correct

B

Only B is correct

C

Both A and B are correct statements

D

Only A is correct

Text Solution

AI Generated Solution

The correct Answer is:
### Step-by-Step Solution: 1. **Understanding Statement A**: - Statement A claims that "Gel electrophoresis and elution are two important processes." - **Explanation**: Gel electrophoresis is a technique used to separate DNA, RNA, or proteins based on their size. It is a crucial method in biotechnology, particularly in applications like DNA fingerprinting, where samples are separated according to their molecular size. Elution, on the other hand, refers to the process of extracting one material from another, typically by washing it with a solvent. This is important for isolating DNA samples for further analysis. 2. **Evaluating Statement B**: - Statement B states that "After staining with ethidium bromide, it has to be exposed to UV light." - **Explanation**: Ethidium bromide is a fluorescent dye that intercalates between the bases of DNA. When exposed to UV light, it fluoresces, allowing visualization of the DNA bands on the gel. This is a standard procedure in molecular biology to detect DNA after gel electrophoresis. 3. **Conclusion**: - Both statements A and B are correct. Gel electrophoresis and elution are indeed important processes in DNA analysis, and the use of ethidium bromide followed by UV exposure is a standard method for visualizing DNA. ### Final Answer: Both Statement A and Statement B are correct. ---
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Which colour would you find in DNA strands after stain- ing with Ethidium bromide in the presence of UV light ?

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reasons?

You can see bright orange-coloured bands of DNA in ethidium bromide stained gel exposed to

Read the following statements. Statement A: In agarose gel electrophoresis, the smaller the fragment size of DNA, the farther it moves from the wall. Statement B: Separation of proteins in normal electrophoresis is based on both size and charge. Choose the correct option.

Some questions (Assertion-Reason Type) are given below. Each question contains Statement I (Assertion) and statement II(reason). Each question has 4 choices (a),(b),(c ) and (d) out of which only one is correct. So select the correct choise. a. Statement I is True, Statement II is True,Statement II is a correct explanation for Statement I b. Statement I is True, Statement II is True, Statement II is NOT a correct ecplanation for Statement I c. Statement I is True, Statement II is False . d. Statement I is false, Statement II is True. Statement:I Though light of a single frequency (monochromatic light) is incident on a metal, the energies of emitted photoelectrons are different. Statement II: The energy of electrons just after they absorb photons incident on the metal surface may be lost in collision with other atoms in the metal before the electron is ejected out of the metal.

Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below

After electrophoresis the separated DNA fragment can be visualized ethidium bromide gel exposed UV light . These DNA fragments appear as _______ coloured bands :

After electrophoresis the separated DNA fragment can be vishualised ethidium bromide gel exposed UV light . These DNA fragments appear as _______ coloured bands :

a) How do DNA fragments migrate and resolve in a Gel electrophoresis? b) How lane one is different from lane 2,3 and 4 in the GEL electrophoresis set up? c) How pure DNA fragments are made observable in the visible light?

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