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Gel electrophoresis is used for...

Gel electrophoresis is used for

A

Cutting DNA

B

Isolation of DNA

C

Construction of recombinant DNA

D

Separation of DNA fragments according to their size.

Text Solution

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The correct Answer is:
D
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Explore conceptually related problems

Read the following statements. Statement A: In agarose gel electrophoresis, the smaller the fragment size of DNA, the farther it moves from the wall. Statement B: Separation of proteins in normal electrophoresis is based on both size and charge. Choose the correct option.

In electrophoresis,

In electrophoresis :-

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

How can DNA segment, separated by gel electrophoresis be visualised and isolated ?

How can DNA segment, separated by gel electrophoresis be visualised and isolated ?

Assertion : The matrix used in gel electrophoresis should have controllable pore size. Reason : Agarose concentration can be changed to change pore sizes.

Explain the basis on which the gel electrophoresis technique works. Write any two ways the products obtained through this technique can be utilized.

DINESH PUBLICATION ENGLISH-PRINCIPLES AND APPLICATIONS OF BIOTECHNOLOGY-RQ
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