Isolation of DNA from a fungal cell involves the use of enzyme

Options

A. Chitinase
B. Lysozyme
C. Eco RI
D. Hind II

Answer

To isolate DNA from a fungal cell, we need to break down the cell wall, which is primarily composed of chitin. The process involves the following steps:

### Step-by-Step Solution:

1. **Understanding the Fungal Cell Structure**:
- Fungal cells have a rigid cell wall made primarily of chitin, which provides structural support. To isolate DNA, we need to disrupt this cell wall.

2. **Identifying the Requirement for Enzymes**:
- To break down the cell wall, specific enzymes that can hydrolyze the components of the cell wall are required.

3. **Analyzing the Options**:
- The options provided are:
- A) Chitinase
- B) Lysozyme
- C) E. coli
- D) Hint 2
- We need to determine which of these enzymes is effective in breaking down chitin.

4. **Choosing the Correct Enzyme**:
- **Chitinase** is an enzyme that specifically breaks down chitin by hydrolyzing the glycosidic bonds present in it.
- **Lysozyme**, on the other hand, primarily acts on peptidoglycan in bacterial cell walls and is not effective against chitin.
- **E. coli** is a bacterium and not an enzyme, thus irrelevant in this context.

5. **Conclusion**:
- Since chitinase is the enzyme that can hydrolyze chitin and facilitate the isolation of DNA from fungal cells, the correct answer is **Option A: Chitinase**.

### Final Answer:
The isolation of DNA from a fungal cell involves the use of the enzyme **chitinase** (Option A).

---