Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322

Options

A. rop, `amp^(R)`
B. `amp^(R)`, rop
C. rop, ori
D. ori, rop

Answer

To answer the question regarding the restriction sites for Pvu I and Pvu II in the pBR322 vector, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Structure of pBR322**:
- pBR322 is a commonly used plasmid vector in genetic engineering.
- It is a double-stranded circular DNA molecule consisting of 4361 base pairs.
- It contains two main selectable markers: one for ampicillin resistance (ampR) and one for tetracycline resistance (tetR).

2. **Identify the Key Genes in pBR322**:
- The key genes present in pBR322 include:
- Ampicillin resistance gene (ampR)
- Tetracycline resistance gene (tetR)
- ROP gene (which controls the copy number of the plasmid)
- Origin of replication (ORI)

3. **Locate the Restriction Sites**:
- The question specifically asks for the restriction sites for Pvu I and Pvu II.
- Pvu I is located in the ampicillin resistance gene (ampR).
- Pvu II is located in the ROP gene.

4. **Analyze the Options Given**:
- The options provided are:
- A) ROP and ampicillin resistant
- B) Ampicillin resistant and ROP
- C) ROP and ORI
- D) ORI and ROP
- Based on our findings:
- Pvu I is in the ampicillin resistance gene (ampR).
- Pvu II is in the ROP gene.

5. **Select the Correct Option**:
- The correct option that matches our findings is:
- **B) Ampicillin resistant and ROP**.

### Final Answer:
The restriction sites for Pvu I and Pvu II respectively are in the **ampicillin resistance gene and the ROP gene** (Option B).

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