Identify the correct sequence of steps in RNA-interference (RNAi) process to develop nematode resistant plants
(i) Silencing of specific mRNA of the nematode
(ii) Formation of sense and antisense RNA
(iii) dsRNA formation
(iv) Introducing nematode specific genes into plant

Options

A. i, ii, iv, iii
B. iii, ii, i, iv
C. iv, ii, iii, i
D. iv, i, ii, iii

Answer

To identify the correct sequence of steps in the RNA interference (RNAi) process to develop nematode-resistant plants, we can break down the process into the following steps:

### Step-by-Step Solution:

1. **Introducing Nematode Specific Genes into Plants (iv)**:
The first step in the RNAi process is to introduce nematode-specific genes into the plant. This is crucial as it provides the genetic material that will be targeted for silencing.

2. **Formation of Sense and Antisense RNA (ii)**:
After the introduction of these genes, the plant cells will begin to transcribe the introduced genes, resulting in the formation of both sense (the original RNA strand) and antisense RNA (the complementary strand).

3. **dsRNA Formation (iii)**:
The sense and antisense RNA strands will then hybridize to form double-stranded RNA (dsRNA). This dsRNA is a stable molecule that is crucial for the RNA interference mechanism.

4. **Silencing of Specific mRNA of the Nematode (i)**:
Finally, the presence of dsRNA leads to the silencing of specific mRNA of the nematode. This occurs because the dsRNA will bind to the target mRNA, preventing it from being translated into proteins, which ultimately helps in resisting the nematode.

### Correct Sequence:
The correct sequence of steps is:
(iv) → (ii) → (iii) → (i)