Most suitable method of introducing alien DNA into a plant cell is

Options

A. Biolistics
B. Micro - injection
C. Lipofection
D. Heat shock method

Answer

### Step-by-Step Solution:

1. **Understanding the Question**: The question asks for the most suitable method to introduce alien (foreign) DNA into a plant cell. This is a common task in biotechnology, especially in genetic engineering.

2. **Reviewing the Options**: The options provided are:
- Biolistic
- Microinjection
- Lipofection
- Heat shock method

3. **Analyzing Each Method**:
- **Biolistic Method**: This method involves using a gene gun to shoot microscopic particles coated with DNA into plant cells. The high velocity of these particles allows them to penetrate the cell wall and membrane, facilitating the introduction of foreign DNA.
- **Microinjection**: This technique involves directly injecting DNA into the plant cell using a fine needle. While effective, it is more labor-intensive and not as widely used for plant cells.
- **Lipofection**: This method uses liposomes to deliver DNA into cells. It is more commonly used in animal cells and is less effective in plant cells due to the rigid cell wall.
- **Heat Shock Method**: This technique involves subjecting cells to a heat shock to facilitate the uptake of DNA. It is often used in bacterial transformation but is not the most effective for plant cells.

4. **Identifying the Most Suitable Method**: After analyzing the methods, the biolistic method stands out as the most suitable for introducing foreign DNA into plant cells due to its efficiency in penetrating the cell wall and membrane.

5. **Final Answer**: The most suitable method of introducing alien DNA into a plant cell is **biolistic**.