The viability of seeds can be ascertained with the help of

Options

A. IAA
B. A.B.A
C. T.T.C.
D. N.A.D.P.

Answer

**Step-by-Step Solution:**

1. **Understanding Seed Viability**: Seed viability refers to the ability of a seed to germinate and develop into a healthy plant. Each seed has a specific time frame in which it must germinate; otherwise, it may lose its viability.

2. **Importance of Viability**: Just like food and medicines have expiry dates, seeds also have a time limit for germination. If they do not germinate within this time, the stored nutrients in the seed may be depleted, leading to the seed's death.

3. **Testing Viability**: To ascertain the viability of seeds, various methods can be used. One effective method is the use of TTC (Triphenyl Tetrazolium Chloride).

4. **Role of TTC**: TTC is a chemical that helps detect the presence of an enzyme called dehydrogenase, which is active in viable seeds. When viable seeds are treated with TTC, the dehydrogenase enzyme converts the colorless TTC into a reddish or pinkish compound called formazan.

5. **Interpreting Results**:
- If the TTC solution turns reddish or pink, it indicates that the seed is viable.
- If the solution turns yellow or bluish, it suggests that the seed may be weak and its viability is uncertain.
- If there is no color change, it indicates that the seed is no longer viable.

6. **Procedure for Testing**: To perform the test, take the seed and cut it in half to expose the embryo. Soak the half with the embryo in TTC overnight. The color change observed the next day will help determine the seed's viability.

7. **Conclusion**: Based on the information provided, the answer to the question "The viability of seeds can be ascertained with the help of" is **TTC**.

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