To isolate DNA from fungi we have to break the wall. This is done by

Options

A. Lysozyme
B. Cellulose
C. Invertase
D. Chitinase

Answer

To isolate DNA from fungi, we need to break down the fungal cell wall, which is primarily composed of chitin. The process involves the following steps:

1. **Understanding the Composition of Fungal Cell Walls**: Fungal cell walls are mainly made up of chitin, a polysaccharide that provides structural support. To access the DNA, we must first break down this cell wall.

2. **Identifying the Appropriate Enzyme**: To break down chitin, we need an enzyme that specifically targets this polysaccharide. The enzyme that fulfills this requirement is chitinase.

3. **Using Chitinase to Break Down the Cell Wall**: By applying chitinase, we can effectively digest the chitin in the fungal cell wall. This enzymatic action will lead to the breakdown of the wall, allowing access to the cytoplasm where the DNA is located.

4. **Isolating the DNA**: Once the cell wall is broken down, the DNA can be isolated from the cytoplasm using standard DNA extraction techniques.

5. **Conclusion**: The correct enzyme to use for breaking down the fungal cell wall to isolate DNA is chitinase.

**Final Answer**: The correct option is D) chitinase.