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Arrange these steps for DNA fingerprinti...

Arrange these steps for DNA fingerprinting in the correct order:
(I) Separated DNA fragments were transferred (blotting technique) to synthetic membranes, such as nitrocellulose or nylon.
(II) Use of autoradiography for detection of hybridised DNA fragments.
(III) DNA isolation: the DNA is isolated from the desired cells.
(IV) Digestion of DNA by restriction endonucleases Order:

A

(I), (II), (III), (IV)

B

(IV), (III), (II), (I)

C

(III), (IV), (I), (II)

D

(II), (III), (IV), (I)

Text Solution

Verified by Experts

The correct Answer is:
C
Steps involved in the technique are:
DNA isolation: the DNA is isolated from the desired cells.
DNA amplification
Digestion of DNA by restriction endonucleases. Agarose gel electrophoresis was used for separation of DNA fragments.
Separated DNA fragments were transferred (blotting technique) to synthetic membranes, such as nitrocellulose or nylon.
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