The enzyme that catalyses phosphorylation of the substrate without using ATP molecule is

Options

A. Glyceraldehyde 3-phosphate dehydrogenase
B. Glucose 6-phosphotransferase
C. Phosphofructokinase
D. Pyruvatedikinase

Answer

To solve the question regarding the enzyme that catalyzes phosphorylation of the substrate without using an ATP molecule, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Question**: The question asks for an enzyme that can catalyze the phosphorylation of a substrate without the involvement of ATP. Phosphorylation typically involves the addition of a phosphate group to a molecule.

2. **Identifying Key Terms**: The term "phosphorylation" is crucial here. It indicates that we are looking for an enzyme that adds a phosphate group to a substrate. The phrase "without using ATP" suggests that the enzyme must utilize another source of phosphate.

3. **Analyzing the Options**:
- **Option A: Glyceraldehyde 3-phosphate dehydrogenase**: This enzyme catalyzes the conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, utilizing inorganic phosphate (Pi) and NAD+ in the process. It does not use ATP.
- **Option B: Glucose 6-phosphor transferase**: This enzyme typically transfers a phosphate group from ATP to glucose, thus it does not fit the criteria of not using ATP.
- **Option C: Pyruvate kinase**: This enzyme catalyzes the transfer of a phosphate group from phosphoenolpyruvate to ADP, generating ATP, which again involves ATP usage.
- **Option D: (Not provided in the transcript)**: We can assume it does not fit the criteria based on the context.

4. **Conclusion**: Based on the analysis, the enzyme that catalyzes phosphorylation of the substrate without using ATP is **Glyceraldehyde 3-phosphate dehydrogenase**.

### Final Answer:
The enzyme that catalyzes phosphorylation of the substrate without using ATP molecule is **Glyceraldehyde 3-phosphate dehydrogenase**.

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