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You can see bright orange-coloured bands...

You can see bright orange-coloured bands of DNA in ethidium bromide stained gel exposed to

A

X-rays

B

sun rays

C

UV rays

D

gamma rays

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The correct Answer is:
C
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Assertion: One can see bright orange coloured band of DNA in a ethidium bromide stained gel exposed to infra-red light Reason :One cannot see pure DNA fragments in the visible & UV light & without staining

With respect to DNA fragmentation Statement A: Gel electrophoresis and elution are two important processes Statement B : After staining with ethidium bromide, it has to be exposed to UV light

After electrophoresis the separated DNA fragment can be visualized ethidium bromide gel exposed UV light . These DNA fragments appear as _______ coloured bands :

After electrophoresis the separated DNA fragment can be vishualised ethidium bromide gel exposed UV light . These DNA fragments appear as _______ coloured bands :

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites,Ori, antibiotic resistance genes& ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

Which colour would you find in DNA strands after stain- ing with Ethidium bromide in the presence of UV light ?

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reasons?

AAKASH SERIES-PRINCIPLES AND PROCESSES -Exercise - III (Previous AIPMT/NEET Questions)
  1. You can see bright orange-coloured bands of DNA in ethidium bromide st...

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  2. DNA fragments are

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  3. The process of separation and purification of expressed protein before...

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  4. The DNA fragments separated on an agarose gel can be visualised after ...

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  5. Stirred - tank bioreactors have been designed for

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  6. A foreign DNA and plasmid cut by the same restriction endonuclease can...

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  7. Which of the following is not component of downstream processing?

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  8. Which of the following restriction enzymes produces blunt ends?

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  9. The taq polymerase enzyme is obtained from

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  10. Which of the following is not required for any of the techniques of DN...

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  11. Which of the following is a restriction endonucleases?

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  12. Golden rice is a genetically modified crop plant where the incorporate...

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  13. An analysis of chromosomal DNA using Southern hybridization technique ...

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  14. In vitro clonal propagation in plants is charcterised by

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  15. Which vector can clone only a small fragment of DNA ?

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  16. DNA fragments generated by the restriction endonucleases in a chemical...

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  17. The colonies of recombinant bacteria apppear white in contrast to blue...

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  18. For transformation, micro- particles coated with DNA to be bombarded w...

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  19. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  20. Agarose extracted from sea weeds finds use in

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  21. Which one of the following is used as vector for cloning genes into hi...

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