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E.coli cloning vector pBR322 contains re...

E.coli cloning vector pBR322 contains restriction sites in the region of ams, `tet^(2)` genes that codes for the:

A

antibiotic resistance genes

B

foreign DNA

C

selection of recombinants from nonrecombinants

D

proteins involved in the replication of the plasmid

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The correct Answer is:
D
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E. coli cloning vector pBR322 contains restriction sites in the region of amp^( R) , tet^(R ) genes that codes for the

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out from agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites,Ori, antibiotic resistance genes& ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

The vector also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contains unique recognition sites region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides:

The diagram shows the structure of a bacterial cell. The presence of structure C in the bacterial cell is one reason why bacteria are used in genetic engineering . In the cloning vector pBR322, which gene is responsible for the replication of structure X ?

(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play. (b) Why is the coding sequence of an enzyme b-galactosidase a preferred selectable marker in comparison to the ones named above ?

P^(BR322) cloning vector has the genes for resistance against

If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants ?

AAKASH SERIES-PRINCIPLES AND PROCESSES -Exercise - III (Previous AIPMT/NEET Questions)
  1. E.coli cloning vector pBR322 contains restriction sites in the region ...

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  2. DNA fragments are

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  3. The process of separation and purification of expressed protein before...

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  4. The DNA fragments separated on an agarose gel can be visualised after ...

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  5. Stirred - tank bioreactors have been designed for

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  6. A foreign DNA and plasmid cut by the same restriction endonuclease can...

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  7. Which of the following is not component of downstream processing?

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  8. Which of the following restriction enzymes produces blunt ends?

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  9. The taq polymerase enzyme is obtained from

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  10. Which of the following is not required for any of the techniques of DN...

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  11. Which of the following is a restriction endonucleases?

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  12. Golden rice is a genetically modified crop plant where the incorporate...

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  13. An analysis of chromosomal DNA using Southern hybridization technique ...

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  14. In vitro clonal propagation in plants is charcterised by

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  15. Which vector can clone only a small fragment of DNA ?

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  16. DNA fragments generated by the restriction endonucleases in a chemical...

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  17. The colonies of recombinant bacteria apppear white in contrast to blue...

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  18. For transformation, micro- particles coated with DNA to be bombarded w...

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  19. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  20. Agarose extracted from sea weeds finds use in

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  21. Which one of the following is used as vector for cloning genes into hi...

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