Cleavage style of restriction enzymes includes

Options

A. blunt end style
B. sticky end style
C. no style
D. Both (1) and (2)

Answer

### Step-by-Step Solution:

1. **Understand the Question**: The question asks about the cleavage styles of restriction enzymes. We need to identify which styles are included.

2. **Identify the Types of Cleavage Styles**: There are generally two main types of cleavage styles for restriction enzymes:
- **Blunt Ends**: These are ends where the cut is straight across both strands of DNA.
- **Sticky Ends**: These are ends where the cut is staggered, resulting in overhanging sequences that can easily pair with complementary sequences.

3. **Analyze the Options Given**:
- **Option 1**: Blunted style - This refers to enzymes that produce blunt ends.
- **Option 2**: Sticky end style - This refers to enzymes that produce sticky ends.
- **Option 3**: No style - This option suggests that there are no cleavage styles, which is incorrect.
- **Option 4**: Both one and two - This option suggests that both blunt and sticky ends are included.

4. **Research on Hind II**: The video mentions the restriction enzyme Hind II, which is known to produce blunt ends. This indicates that at least one type of cleavage style is confirmed.

5. **Conclusion**: Since restriction enzymes can produce both blunt ends and sticky ends, the correct answer would be **Option 4: Both one and two**. However, since the video specifically emphasizes Hind II producing blunt ends, it may lead to some confusion.

### Final Answer:
The cleavage style of restriction enzymes includes **both blunt end style and sticky end style** (Option 4).

---