Match each term column-I with the definition in column-II,
Column - I
A) Bacterial enzymes used to cut DNA at defined sequences
B) Sequences cut by restriction enzymes
C) Ends left on DNA segments cut by DNA restriction enzymes
D) Circular pieces of DNA found in bacteria
E) Bacterial viruses
F) Process by which bacteria take up pieces of DNA from the environment
G) Plasmid DNA that has incorporated human DNA
H) Term used to describe any vehicle that moves DNA from the organism to another
Column - II
I) Transformation
II) Cloning vector
III) Recombinant
IV) Recognition sequences
V) Plasmids
VI) Sticky ends
VII) Restriction enzymes
VIII) Bacteriophages

Options

A. A-VII,B-IV,C-VI,DI-V,E-VIII,F-I,G-III,H-II`
B. A-IV,B-VI,C-I,D-III,E-V,F-VII,G-VIII,H-II
C. A-III,B-V,C-VII,D-VIII,E-II,F-IV,G-VI,H-I
D. A-IV,B-III,C-V,D-VI,E-VII,F-I,G-VIII,H-II

Answer

To match each term in Column-I with the definition in Column-II, we will analyze each term and find the corresponding definition step by step.

### Step-by-Step Solution:

1. **A) Bacterial enzymes used to cut DNA at defined sequences**
- These enzymes are known as **Restriction enzymes**. They are specialized proteins that recognize specific sequences in DNA and cut the DNA at these sites.
- **Match:** A → VII (Restriction enzymes)

2. **B) Sequences cut by restriction enzymes**
- The specific sequences where restriction enzymes cut DNA are referred to as **Recognition sequences**. These are the target sequences recognized by the enzymes.
- **Match:** B → IV (Recognition sequences)

3. **C) Ends left on DNA segments cut by DNA restriction enzymes**
- After the DNA is cut by restriction enzymes, the ends that remain can be either blunt or **Sticky ends**. Sticky ends are single-stranded overhangs that can easily bind with complementary DNA strands.
- **Match:** C → VI (Sticky ends)

4. **D) Circular pieces of DNA found in bacteria**
- The circular DNA molecules present in bacteria are called **Plasmids**. These are independent of chromosomal DNA and can replicate independently.
- **Match:** D → V (Plasmids)

5. **E) Bacterial viruses**
- Viruses that specifically infect bacteria are known as **Bacteriophages**. They can hijack bacterial machinery to replicate themselves.
- **Match:** E → VIII (Bacteriophages)

6. **F) Process by which bacteria take up pieces of DNA from the environment**
- The process through which bacteria can take up foreign DNA from their surroundings is called **Transformation**. This is a key mechanism of horizontal gene transfer.
- **Match:** F → I (Transformation)

7. **G) Plasmid DNA that has incorporated human DNA**
- When plasmid DNA incorporates human DNA, it is referred to as **Recombinant DNA**. This is often used in genetic engineering to produce desired traits.
- **Match:** G → III (Recombinant)

8. **H) Term used to describe any vehicle that moves DNA from one organism to another**
- The term for a vehicle that carries DNA from one organism to another is called a **Cloning vector**. This can be a plasmid, virus, or any other means used to transfer genetic material.
- **Match:** H → II (Cloning vector)

### Final Matches:
- A → VII (Restriction enzymes)
- B → IV (Recognition sequences)
- C → VI (Sticky ends)
- D → V (Plasmids)
- E → VIII (Bacteriophages)
- F → I (Transformation)
- G → III (Recombinant)
- H → II (Cloning vector)

### Conclusion:
The correct option that matches all the pairs is **Option A**.