`P^(BR322)` cloning vector has the genes for resistance against

Options

A. Chloramphenicol and Ampicillin
B. Chloramphenicol and Tetracycline
C. Ampicillin and Tetracycline
D. Streptomycin and Penicillin

Answer

To answer the question regarding the PBR322 cloning vector and the genes it carries for antibiotic resistance, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the PBR322 Vector**:
- PBR322 is a well-known plasmid used as a cloning vector in molecular biology. It is designed to carry foreign DNA into a host cell for cloning purposes.

2. **Identify the Size of PBR322**:
- The PBR322 vector has a size of 4361 base pairs. This size is important as it determines the amount of foreign DNA it can carry.

3. **Examine the Resistance Genes**:
- PBR322 contains specific genes that confer resistance to certain antibiotics. The two primary antibiotic resistance genes present in PBR322 are:
- **Ampicillin (amp)**: This gene provides resistance against the antibiotic ampicillin.
- **Tetracycline (tet)**: This gene provides resistance against the antibiotic tetracycline.

4. **Analyze the Options Given**:
- The options provided in the question are:
- **Chloramphenicol and ampicillin**
- **Chloramphenicol and tetracycline**
- **Ampicillin and tetracycline**
- **Streptomycin and penicillin**
- From our understanding, PBR322 does not contain genes for chloramphenicol or streptomycin resistance.

5. **Select the Correct Answer**:
- Based on the information, the correct answer is that PBR322 has genes for resistance against **ampicillin and tetracycline**.

### Final Answer:
The PBR322 cloning vector has the genes for resistance against **ampicillin and tetracycline**.

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