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The DNA fragments separated on an agaros...

The DNA fragments separated on an agarose gel can be visualised after staining with:

A

Ehthidium bromide

B

Bromophenol blue

C

Acetocarmine

D

Aniline blue

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The correct Answer is:
To solve the question regarding the visualization of DNA fragments separated on an agarose gel, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Process**: Agarose gel electrophoresis is a technique used to separate DNA fragments based on their size and mass. After the separation, it is important to visualize the DNA fragments to analyze the results. 2. **Identify Staining Options**: The question provides four options for staining the DNA fragments: - A) Ethidium bromide - B) Bromophenol blue - C) Aceto carmine - D) Aniline blue 3. **Evaluate Each Stain**: - **Ethidium Bromide**: This is a fluorescent dye that intercalates between the bases of DNA. When exposed to ultraviolet (UV) light, it fluoresces, allowing for the visualization of DNA bands on the gel. - **Bromophenol Blue**: This is a tracking dye commonly used in gel electrophoresis but does not specifically stain DNA for visualization. - **Aceto Carmine**: This is a stain used for visualizing chromosomes in cytological preparations, not typically used for DNA in gels. - **Aniline Blue**: This is a dye used for staining collagen and does not effectively stain DNA in gels. 4. **Select the Correct Answer**: Based on the evaluation, ethidium bromide is the most suitable option for visualizing DNA fragments on an agarose gel after electrophoresis. ### Conclusion: The correct answer to the question is **Option A: Ethidium Bromide**. ---
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AAKASH SERIES-PRINCIPLES AND PROCESSES -Exercise - III (Previous AIPMT/NEET Questions)
  1. DNA fragments are

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  2. The process of separation and purification of expressed protein before...

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  3. The DNA fragments separated on an agarose gel can be visualised after ...

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  4. Stirred - tank bioreactors have been designed for

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  5. A foreign DNA and plasmid cut by the same restriction endonuclease can...

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  6. Which of the following is not component of downstream processing?

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  7. Which of the following restriction enzymes produces blunt ends?

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  8. The taq polymerase enzyme is obtained from

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  9. Which of the following is not required for any of the techniques of DN...

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  10. Which of the following is a restriction endonucleases?

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  11. Golden rice is a genetically modified crop plant where the incorporate...

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  12. An analysis of chromosomal DNA using Southern hybridization technique ...

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  13. In vitro clonal propagation in plants is charcterised by

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  14. Which vector can clone only a small fragment of DNA ?

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  15. DNA fragments generated by the restriction endonucleases in a chemical...

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  16. The colonies of recombinant bacteria apppear white in contrast to blue...

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  17. For transformation, micro- particles coated with DNA to be bombarded w...

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  18. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  19. Agarose extracted from sea weeds finds use in

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  20. Which one of the following is used as vector for cloning genes into hi...

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