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Gel electrophoresis is used for...

Gel electrophoresis is used for

A

construction of recombinant DNA by joining with cloning vectors

B

isolation of DNA molecule

C

cutting of DNA into fragments

D

separation of DNA fragments according to their size

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The correct Answer is:
D
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Explore conceptually related problems

Read the following statements. Statement A: In agarose gel electrophoresis, the smaller the fragment size of DNA, the farther it moves from the wall. Statement B: Separation of proteins in normal electrophoresis is based on both size and charge. Choose the correct option.

In electrophoresis,

In electrophoresis :-

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

How can DNA segment, separated by gel electrophoresis be visualised and isolated ?

How can DNA segment, separated by gel electrophoresis be visualised and isolated ?

Assertion : The matrix used in gel electrophoresis should have controllable pore size. Reason : Agarose concentration can be changed to change pore sizes.

Explain the basis on which the gel electrophoresis technique works. Write any two ways the products obtained through this technique can be utilized.

AAKASH SERIES-PRINCIPLES AND PROCESSES -Exercise - III (Previous AIPMT/NEET Questions)
  1. Which of the following restriction enzymes produces blunt ends?

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  2. The taq polymerase enzyme is obtained from

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  3. Which of the following is not required for any of the techniques of DN...

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  4. Which of the following is a restriction endonucleases?

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  5. Golden rice is a genetically modified crop plant where the incorporate...

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  6. An analysis of chromosomal DNA using Southern hybridization technique ...

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  7. In vitro clonal propagation in plants is charcterised by

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  8. Which vector can clone only a small fragment of DNA ?

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  9. DNA fragments generated by the restriction endonucleases in a chemical...

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  10. The colonies of recombinant bacteria apppear white in contrast to blue...

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  11. For transformation, micro- particles coated with DNA to be bombarded w...

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  12. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  13. Agarose extracted from sea weeds finds use in

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  14. Which one of the following is used as vector for cloning genes into hi...

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  15. Which one of the following palindromic base sequences in DNA can be ea...

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  16. DNA or RNA segment tagged with a radioactive molecule is called

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  17. Restriction endonucleases are enzymes which

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  18. Polyethylene glycol method is used for :

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  19. Gel electrophoresis is used for

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  20. The linking of antibiotic resistance gene with the plasmid vector beca...

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