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RNA primer...

RNA primer

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1 RNA primer attracts the complementary nucleotides from the surrounding nucleoplasam
2 It helps in building new complimentary strand of m RNA
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Consider the following events in DNA replication 1. Formation of RNA primers by RNA polymerase. 2. Removal of RNA primers and formation of Okazaki fragments. Formation of DNA strands on RNA primers by DNA polyIII complex. 4. Recognition of intiation point by initiator protein. 5. Dissociation of hydrogen bonds and opening of a 'bubbble' in the duplex by unwinding proteins. 6. Filling up of the gaps between Okazaki fragments and formation of DNA strand. ltBrgt the correct sequence of these events is:-

Match the columns and select the correct option column-I a. Kornberg enzyme b. DNA ligase c. Snurps d. Peptidyl transferase column-II (i) removal of introns (ii) ribozyme (iii) joining of okazaki fragments (iv) removal of RNA primers

The replication process involves the following steps: (I) The enzyme DNA-dependent DNA poly merases catalyse the polymerisation of DNA strand. (II) The lagging strand is synthesized in frag ments known as Okazaki fragments, which are later joined by the enzyme DNA Ligase. (III) The origin of replication serves as the recognition site for DNA polymerase and also it provides the site for attachment of RNA primer. (IV)The structural gene coding for a partic ular protein is flanked by the promoter and terminator. Choose the correct option:

Select the correct option that correctly fill the blanks i-iv. I . Less than (i)____ of genome represents structural genes that code for proteins. ii. Chemical substance that binds with repressor and convert it into a non-DNA binding state is (ii)_____. III. In prokaryotes, during replication RNA primer is removed by (iii) where as in eukaryotes it is removed by(Iv)______.

Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the hight temperature induced denaturation of dsDNA. (ii) It required primers for carrying out the process of polymerisation. It synthesises the RNA region between the primers, using dNTPs and Mg^(2+) .

Which polymerase erases primer and fills gap?

Gene amplification using primers can be done by :

During PCR technique the pairing of primers to ssDNA segment is called

During polymerase chain reaction , which primers are used for initiating the process .