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RNA interference is a technique used for...

RNA interference is a technique used for making tobacco plants resistant against pest like nematode Meloidegyne incognita . In this technique , which of the following processes is inhibited ?

A

Transcription of mRNA

B

Maturation of hn RNA.

C

Translation of mRNA

D

Replication of DNA

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The correct Answer is:
To solve the question regarding RNA interference (RNAi) and its role in making tobacco plants resistant to the nematode Meloidogyne incognita, we need to analyze the options provided and understand the mechanism of RNA interference. ### Step-by-Step Solution: 1. **Understanding RNA Interference (RNAi)**: - RNA interference is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules. - It is a method of cellular defense that helps in silencing specific mRNA. 2. **Mechanism of RNAi**: - In RNAi, double-stranded RNA (dsRNA) is introduced into the plant cells. - This dsRNA is complementary to the target mRNA (in this case, mRNA from the nematode). - The dsRNA binds to the specific mRNA and prevents its translation into proteins. 3. **Analyzing the Options**: - A. **Transcription of mRNA**: This process involves synthesizing mRNA from DNA. RNAi does not inhibit transcription. - B. **Maturation of hnRNA**: This refers to the processing of heterogeneous nuclear RNA into mature mRNA. RNAi does not directly inhibit this process. - C. **Translation of mRNA**: This is the process where ribosomes synthesize proteins based on the mRNA sequence. RNAi specifically inhibits this process by preventing the translation of the target mRNA. - D. **Replication of DNA**: This is the process of copying DNA. RNAi does not inhibit DNA replication. 4. **Conclusion**: - The correct answer is **C. Translation of mRNA**. RNA interference primarily inhibits the translation process, thereby preventing protein synthesis in the target organism (in this case, the nematode). ### Final Answer: **C. Translation of mRNA**
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