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Separation and isolation of DNA fragment...

Separation and isolation of DNA fragments is a major event in recombi-nant DNA technology . The separated fragments cannot be seen in a nor-mal way . Is there any method to visualise the separated DNA ? Explain .

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The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation . DNA can be seen as bright orange coloured bands.
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NEW JOYTHI PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -New Evaluation Type Questions
  1. Palindromic nucleotide sequences have significance in recombinant DNA ...

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  2. During genetic engineering DNA fragments are produced by cutting DNA w...

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  3. Separation and isolation of DNA fragments is a major event in recombi-...

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  4. How are the DNA fragments separated through gel electrophoresis ex-tra...

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  5. Name two important cloning vectors .

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  6. What are the main features that are required to facilitate cloning in...

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  7. Explain the significance of origin of replication and selectable marke...

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  8. Name any three suitable 'selectable marker' for E.coli.

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  9. During genetic engineering experiments recombinants are distinguished ...

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  10. DNA is a hydrophilic molecule, so it cannot pass through cell membrane...

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  11. Number of recognition sites usually preferred for a vector is

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  12. You are provided with the mixture of transformed bacteria having recom...

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  13. Agrobacterium tumifaciens act as a natural genetic engineer , who tran...

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  14. Given below are the different steps in recombinant DNA technology. Arr...

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  15. DNA is usually intertwind with histone proteins and RNA. But in geneti...

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  16. Briefly explaing the 'cutting' and 'ligation' of DNA during recombinan...

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  17. One of the methods by which recombinant DNA can be introduced into hos...

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  18. Explain how the gene of interest is amplified invitro during biotechno...

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  19. What is the significance of thermostable DNA polymerase during PCR ? ...

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  20. Distinguish between recombinant DNA and recombinant protein.

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