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Name any three suitable 'selectable mark...

Name any three suitable 'selectable marker' for E.coli.

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The genes encoding resistance to antibiotics such as ampicillin, chloram-phenicol, tetracycline are suitable, .selectable markers. for E.coli.
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If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as 1) selectable marker 2) recombinant protein 3) cloning site 4) chemical scalpels

NEW JOYTHI PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -New Evaluation Type Questions
  1. Separation and isolation of DNA fragments is a major event in recombi-...

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  2. How are the DNA fragments separated through gel electrophoresis ex-tra...

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  3. Name two important cloning vectors .

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  4. What are the main features that are required to facilitate cloning in...

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  5. Explain the significance of origin of replication and selectable marke...

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  6. Name any three suitable 'selectable marker' for E.coli.

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  7. During genetic engineering experiments recombinants are distinguished ...

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  8. DNA is a hydrophilic molecule, so it cannot pass through cell membrane...

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  9. Number of recognition sites usually preferred for a vector is

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  10. You are provided with the mixture of transformed bacteria having recom...

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  11. Agrobacterium tumifaciens act as a natural genetic engineer , who tran...

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  12. Given below are the different steps in recombinant DNA technology. Arr...

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  13. DNA is usually intertwind with histone proteins and RNA. But in geneti...

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  14. Briefly explaing the 'cutting' and 'ligation' of DNA during recombinan...

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  15. One of the methods by which recombinant DNA can be introduced into hos...

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  16. Explain how the gene of interest is amplified invitro during biotechno...

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  17. What is the significance of thermostable DNA polymerase during PCR ? ...

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  18. Distinguish between recombinant DNA and recombinant protein.

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  19. Give the significance of using a 'bioreactor' in biotechnological expe...

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  20. Explain the importance of (a) ori, (b) amp^(R) and (c) rop in the E. c...

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